Brdu flow kits instruction manual

For Use With (Application) For immunofluorescent BrdU staining, fluorescent Anti-cytokine antibodies in analyses of cultured cells. For Use With (Equipment) Flow cytometer. Includes. One 65μL vial of APC-conjugated Anti-BrdU antibody, one 25mL bottle Cytofix/Cytoperm™ buffer, two 25mL bottles perm/Wash™ buffer (10X), one 10mL bottle. Kit Size Cat. No. BrdU In-situ Detection Kit 50 slides Biotinylated Anti-BrdU Antibody ml L Diluent Buffer 15 ml KD BD™ Retrievagen A, Solution 1 10 ml KD BD™ Retrievagen A, Solution 2 50 ml KD Fixation Buffer 10 ml KD DAB Chromogen ml KD DAB Buffer 10 ml KD. BD Pharmingen FITC BrdU Flow Kit (FITC-conjugated anti-BrdU, BD Cytofix/ Cytoperm™ Buffer, BD Perm/Wash™ Buffer (10X), BD Cytoperm™ Permeabi-lization Buffer Plus, 7-AAD) Hu, Ms IC/FCM: IC/FCM FITC: FITC 50 tests: 4 x

Flow Cytometry – BestProtocols® Page 1 of 3 BrdU Staining Kit for Flow Cytometry Research Use Only For additional questions, please contact Technical Support at + (US) or +43 1 (Europe/International), or send us an email at Tech_Support@www.doorway.ru BrdU Flow Kits Instruction Manual FITC BrdU Flow Kit Catalog No. (50 tests) Catalog No. (4 x 50 tests) APC BrdU Flow Kit Catalog No. (50 tests). Keep the fluorochrome-conjugated anti-BrdU antibody and 7-AAD protected from light. Unused portions of 1X BD Cytofix/Cytoperm Buffer can be stored at 2 to 8°C. BrdU and DNase. Store the BrdU and DNase at –80°C. The BrdU solution has been shown to be stable for up to 4 months at 2 to 8°C, or it can be refrozen.

Analyze cells by flow cytometry following the manufacturer's instructions. The DNA stain should be read on the appropriate channel set to the height and linear. BrdU was injected IP at the time of infection, and mice were sacrificed 24 hours later. We followed the kit per instructions and obtained. Storage of Kit Components. The Exalpha Biologicals BrdU Fluorescent. Immunohistochemistry Kit components are shipped on ice packs.