Glutathione sepharose 4b manual

There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B well plates. For sample volumes between µl and 10 ml use GST GraviTrap™ gravity flow column. Glutathione Sepharose 4B is designed for purification of glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, other glutathione S-transferases, and glutathione binding proteins. GST-tagged proteins can be purified directly from pretreated bacterial lysates with a one-step method using GSTrap 4B. Glutathione Sepharose 4B required for your application. Glutathione Sepharose 4B as supplied is approximately a 75% slurry. The following procedure results in a 50% slurry. • Gently shake the bottle of Glutathione Sepharose 4B to resuspend the gel. • Use a pipet to remove sufficient slurry for use and transfer to the Disposable www.doorway.ru Size: KB.

Glutathione Sepharose™ 4B, 10 ml Section 5. Fire-fighting measures Extinguishing media Suitable Use dry chemical, CO2, water spray (fog) or foam. Not suitable Do not use water jet. Specific hazards arising from the chemical Flammable liquid and vapor. In a fire or if heated, a pressure increase will occur and the container may. Comparison of Glutathione Sepharose High Performance with Glutathione Sepharose 4 Fast Flow. Note: Only the eluted peaks are shown. mAU 0 ml HP FF GSTrap HP - prepacked Glutathione Sepharose High Performance columns Glutathione Sepharose High Performance is available in. • Glutathione Sepharose 4B provides the highest binding capacity and recovery Fig 1. Glutathione Sepharose affinity chromatography media are available in a variety of different pack sizes as well as in prepacked GSTrap (1 and 5 ml) and GSTPrep FF 16/10 columns. • Glutathione Sepharose 4 FF has improved flow properties.

Glutathione Sepharose 4B is also available in ml and. ml pack sizes. Manual purification with GSTrap 4B columns is easily. GST MicroSpin Purification Module, GSTrap FF, GSTPrep FF 16/10,. Glutathione Sepharose 4 Fast Flow, Glutathione Sepharose 4B. Instructions AK. Affinity media. GE Healthcare. Glutathione Sepharose 4B. Glutathione Sepharose™ 4B is designed for the single-step purification.